ABSTRACT
The aim of these experiments were conducted to study the effect of direct application of the palm kernel meal containing probiotic in the feces of native chicken to reduce the fecal ammonia emission. The experiments were conducted in laboratory. Palm kernel meal as sources of prebiotic with six culture of probiotic bacteria such as B. subtilis, B. cereus, B. thuringiensis, L. acidophillus, L. bugaricus and S. thermophillus were used in the this experiment . In the this experiment, for each treatment-replication, 50 g of fecal material were collected from the house of native chicken and placed in 400 ml beakers. The fecal sample was then treated with 2%, 4%, 6%, 8% containing palm kernel containing probiotic and covered with plastic wraps. The volatile ammonia and excreta pH were measured after twenty four hours of standing and repeated at 48 hr. Ammonia emissions were measured using Kitagawa Toxic Gas Detector (Kitagawa, aspirating Gas Pump AP-20). Experimental design used was Complete Randomized Design (CRD) consisting of five treatments with five replications. The result of the experiment indicated that the palm kernel meal containing of six bacterial cultures probiotics and three of best combination significantly (P<0,05) reduced the fecal ammonia , excreta pH and moisture content of native chicken. The result of this experiment also showed that the application palm kernel meal containing probiotic were significantly (P<0,05) increased the Bacillus spp. counts in the fecal of native chicken. The conclusion of these experiments were that the direct application the palm kernel meal containing of probiotic in the fresh fecal were effective to reduce ammonia emission in the fecal of native chicken.
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